设为首页 - 网站地图 中国最大的生命科学技术资讯门户

Tel:010-86468961-819
17338135791


扫我下载
您的当前位置:主页 > 期刊文档 > Plant Cell > 正文

中科院取得水稻H3K9甲基化研究新进展

来源:未知 编辑:admin 发布时间:2017-08-15

最近,来自中科院遗传和发育研究所、山东师范大学、中国水稻研究所的研究人员发现水稻(SDG714)编码组蛋白H3K9特异的转甲基酶(methyltransferase)。结果提示在水稻中,由SDG714介导的组蛋白H3K9甲基化与DNA甲基化、转位因子的转位和基因组稳定性有关。研究结果刊登于1月26日《plant Cell》在线版上。

水稻(Oryza sativa)H3K9的甲基化功能未知,Arabidopsis thaliana中由于Kryptonite(SU(VAR)3-9 homolog同系物)突变引起的组蛋白H2K9甲基化失败,基因组范围(genome-wide)DNA甲基化水平下降,转位因子的转录水平上升。

SDG714的C末端具有酶活性和底物特异性,N末端起核定位作用。RNAi得到的突变SDG714(SDG714IR转化株)与对照组相比,表形无毛,颖、叶子和茎缺少大型分泌物(macro trichomes)。这些突变体中,CpG和CNG胞嘧啶甲基化以及Tos17位点(一个copia样的反转录转位因子,广泛用于研制水稻突变体)的H3K9甲基化程度下降。有意思的是,失去功能的SDG714可以增强转录、引起Tos17转位。总之,这些结果提示在水稻中,由SDG714介导的组蛋白H3K9甲基化与DNA甲基化、转位因子的转位和基因组稳定性有关。

原文摘要:

SDG714, a Histone H3K9 Methyltransferase, Is Involved in Tos17 DNA Methylation and Transposition in RICE

Although the role of H3K9 methylation in rice (Oryza sativa) is unclear, inArabidopsis thaliana the loss of histone H3K9 methylation by mutation of Kryptonite [also known as SU(VAR)3-9 homolog] reduces genome-wide DNA methylation and increases the transcription of transposable elements. Here, we report that rice SDG714 (for SET Domain Group Protein714) encodes a histone H3K9-specific methyltransferase. The C terminus of SDG714 confers enzymatic activity and substrate specificity, whereas the N terminus localizes it in the nucleus. Loss-of-function mutants of SDG714 (SDG714IR transformants) generated by RNA interference display a mostly glabrous phenotype as a result of the lack of macro trichomes in glumes, leaves, and culms compared with control plants. These mutants also show decreased levels of CpG and CNG cytosine methylation as well as H3K9 methylation at the Tos17 locus, a copia-like retrotransposon widely used for the generation of rice mutants. Most interestingly, loss of function of SDG714 can enhance transcription and cause the transposition of Tos17. Together, these results suggest that histone H3K9 methylation mediated by SDG714 is involved in DNA methylation, the transposition of transposable elements, and genome stability in rice.

作者:weiyanjie 点击:

本站所有产品皆来自以下厂商授权
  • www.bio5.cn

相关推荐:

网友评论:

发表评论
请自觉遵守互联网相关的政策法规,严禁发布色情、暴力、反动的言论。
评价:
表情:
用户名: 验证码:点击我更换图片

生物屋,生命科学技术资讯门户

Copyright © 2002-2017 生物屋 版权所有

Top